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. 2020 Jun 19;8:e9203. doi: 10.7717/peerj.9203

Figure 5. DAXX mediated high Pi-induced HUVECs apoptosis through activating ERK signaling.

Figure 5

(A–C) HUVECs were overexpressed with DAXX in the presence or absence of U0126 (100 ng/mL), and then cell apoptosis was assessed using flow cytometric analysis. (D)The apoptosis rate was quantified by FACS software, and data are represented as the mean ± SEM of at least three independent experiments. (E and F) HUVECs were overexpressed with DAXX in the presence or absence of U0126 (100 ng/mL), and then the protein level of cleaved caspase-3 was assessed using western blot analysis. *p < 0.05 compared to control group; **p < 0.01 compared to control group; #p < 0.05 compared to the overexpression DAXX group.