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. 2020 Jun 19;8:e9387. doi: 10.7717/peerj.9387

Figure 4. The hemolymph of blue E. cyaneus or teal E. vittatus possesses significantly higher amounts of putative crustacyanin analogs than the hemolymph of orange or yellow animals.

Figure 4

(A–K) show the data for E. cyaneus, while (L–R) show the data for E. vittatus. (A and L) Representative photographs of animals of different color morphs. (B, C, M, and N) Representative photographs of the extracted hemolymph; in each case, the photograph is placed below the photograph of the animal of the same color morph. (D) Correlation between pereon and hemolymph color (quantified as the R/B value) of 15 E. cyaneus individuals (E, F, O, and P) Representative 2D-PAGE showing the difference in optical density of putative crustacyanin analogs. The hemolymph of ten individuals of E. cyaneus or four individuals of E. vittatus was pooled in each gel. Percent values correspond to the relative abundance of the protein spot. (G, H, and I) A representative 1D-PAGE and densitometry results for the 15-kDa and 25-kDa putative crustacyanin analog bands, respectively, for 12 replicates for the bands in E. cyaneus. Uncropped gels are available in Fig. S3. **, p < 0.01. (J, K, Q, and R) Tandem mass spectrometry (MS/MS) peptide coverage of the 25-kDa and 15-kDa putative crustacyanin analogs for E. cyaneus and E. vittatus.