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. 2020 Jun 21;8(1):e000622. doi: 10.1136/jitc-2020-000622

Figure 4.

Figure 4

Radiotherapy (RT) enhances the antitumor effect of Duox1−/− proinflammatory macrophages. (A) Macrophage precursors from WT or Duox1−/− bone marrow were cultured in the presence of GM-CSF to induce proinflammatory bone marrow–derived macrophages (BMDMs). After 6 days, the proinflammatory BMDMs were injected intratumorally into MC38 or TC1/Luc subcutaneous tumors 1 day after local tumor irradiation (RT) at 8 Gy. (B and E) Tumor growth in wild-type (WT) mice treated with RT+PBS, RT+WT proinflammatory BMDMs or RT+Duox1−/− proinflammatory BMDMs was monitored in MC38 and TC1/Luc tumors, respectively. (C and F) Kaplan-Meier survival curves for treated mice are shown in MC38 and TC1/Luc tumors, respectively. (D and G) Tumor growth is shown for individual mice in each treatment group in MC38 and TC1/Luc tumors, respectively. Data were obtained from three independent experiments (MC38 tumor model, n=13–14) or two independent experiments (TC1/Luc tumor model, n=18–19) and are represented as the mean±SEM. **p<0.01, ***p<0.001, ****p<0.0001 (two-way ANOVA).