Figure 1.

Effects of MH, EDA and Bru on neuronal survival in the CA1 region of the hippocampi after MCAO. (A) The neurological deficit scores of the rats in different groups were evaluated by a five-point scale 24 h after reperfusion. 10 mice were in each group. (B) Quantitative analyses of neuronal survival in the hippocampal CA1 pyramidal layer. The density of the surviving neurons was quantified as percentage changes against the sham control group. ^***P<0.001 vs. sham. ^###P<0.001 vs. MCAO. ^{^^}P<0.01 and ^{^^^}P<0.001 vs. MCAO + MH + EDA. (C) Representative images of immunofluorescence labeling for NeuN in the ischemic hippocampus at day 3 after MCAO; n=10 mice per group. Scale bar: 40 µm. Magnification, x40. MCAO, middle cerebral artery occlusion; MH, mild hypothermia; EDA, edaravone; Bru, brusatol.