Figure 5.

Experimental validation of RNA-seq data in HeLa and CaSki cells. (A and B) Quantitative PCR validation of selected 46 genes and FAM83A in (A) HeLa and (B) CaSki cells with or without FAM83A knockdown. The relative mRNA levels of individual gene transcripts are presented as fold-change of FAM83A knockdown group compared with the si-NS group. The experiments were performed in triplicate. NS, not significant; ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. si-NS. FAM83A, family with sequence similarity 83 member A; si, small interfering RNA; si-NS, scrambled control.