Figure 6.
Effects of curcumin, B(Cur)2 and Fe(Cur)3 on the relative gene expression of ion channels in MDA-MB-231 cells. MDA-MB-231 cells were untreated (control) or treated with curcumin (25 µM), B(Cur)2 (35 µM) or Fe(Cur)3 (8 µM) for 24 h. Total RNA was extracted, converted to cDNA and subjected to reverse transcription-quantitative PCR. The relative gene expression levels of (A) potassium and (B) calcium, chloride and sodium channels was evaluated. Data were presented as the means (95% confidence interval) of 3 biological replicates. The relative expression of all genes presented here was significantly different compared with untreated control (*P<0.05). B(Cur)2, boron-curcumin; Fe(Cur)3, iron-curcumin.