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ERK and PI3K/AKT signaling pathway are activated by LPS stimulation. A549 cells were treated with LPS (1 µg/ml) for 15 and 30 min or 1, 2 and 4 h prior to western blotting. (A) Representative images of the blots showing ERK1/2 and AKT phosphorylation. Semi-quantitative densitometric analysis measuring relative (B) p-ERK1/2 and (C) p-AKT levels following treatment with/without LPS. *P<0.05 and **P<0.01 vs. con. LPS, lipopolysaccharides; Con, control.
