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. 2020 May 19;57(2):456–465. doi: 10.3892/ijo.2020.5068

Figure 5.

Figure 5

LPS-induced ERK and PI3K/AKT signaling activation can be blocked by their corresponding pharmacological inhibitors. A549 cells were first treated with 1 µg/ml LPS and the MEK inhibitor PD98059 or PI3K inhibitor LY294002 at the indicated concentrations prior to western blotting analysis. (A) Representative images of the blots showing ERK1/2 phosphorylation and corresponding semi-quantitative densitometric analysis showing relative p-ERK1/2 levels following respective treatments. (B) Representative images of the blots showing AKT phosphorylation and corresponding semi-quantitative densitometric analysis showing relative p-AKT levels following respective treatments. Data are presented as the mean ± SD. ***P<0.001 vs. con and #P<0.05 vs. LPS. LPS, lipopolysaccharide; con, control.