Figure 5.

The circ-0070934/miR-1236-3p regulatory axis is crucial for the expression of HOXB7. (A and B) After CSCC cells were transfected with circ-0070934 siRNAs with or without miR-1236-3p inhibitors, the mRNA and protein expression levels of HOXB7 mRNA and protein were determined, with GAPDH used as a control. (C and D) After CSCC cells were transfected with miR-1236-3p mimics with or without circ-0070934 overexpression plasmids, the mRNA and protein expression levels of HOXB7 were detected. All experiments were conducted in triplicate. Data are reported as the mean ± SD. ^**P<0.01. CSCC, cutaneous squamous cell carcinoma; circ, circular RNA; miR, microRNA; HOX7B, homeobox 7B; siRNA, small interfering RNA; NC, negative control; inh, inhibitor; OE, overexpression plasmid.