Figure 8.

Representative micrographs illustrating, on the coronal plane, the negligible expression of PK-resistant α-syn immunoreactivity at seven, nine, and 12 months postinjection in the entorhinal cortex (A–C, insets shown in A’–C’) of control rats. Progressive α-syn aggregation after injection of recombinant mouse α-syn fibrils into the hippocampus. A strong PK-resistant α-syn immunoreactivity occurring as both LB-like (arrows) and LN-like (arrowheads) deposits was also found at seven, nine, and 12 months postinjection in the entorhinal cortex (D–F, insets shown in D′–F′) of injected rats. Box-whiskers plots illustrate the stereologically estimated numbers at different time points ± min to max value of LB-like (G) and LN-like (H) inclusions in the two entorhinal cortex of the injected rats. Scale bar: 500 μm (in A, D for A–C, D–F) and 100 μm (in A’, D’ for A’–C’, D’–F’). Asterisks in G, H indicate significant time-dependent changes at p < 0.05.