Table 2.

Recoveries of OTA tested by the Nb-AviTag-based BA-ELISA

Matrix	OTA spiked (μg/kg)	Mean ± SD (μg/kg)	Recovery (%)	RSD (%)
Intra-assay (n = 3)a
barley	3	3.5 ± 0.12	117	3.4
	5	4.2 ± 0.060	84	1.4
	10	10 ± 0.14	100	1.4
	20	25 ± 0.72	125	2.9
oats	3	3.1 ± 0.020	103	0.64
	5	4.8 ± 0.11	96	2.3
	10	8.8 ± 0.28	88	3.2
	20	23 ± 0.45	115	1.9
rice	3	4.1 ± 0.060	137	1.5
	5	5.2 ± 0.18	104	3.5
	10	9.2 ± 0.42	92	4.6
	20	20 ± 0.50	100	2.5
Inter-assay (n = 3)b
barley	3	3.4 ± 0.050	113	1.5
	5	4.4 ± 0.050	88	1.1
	10	10 ± 0.34	100	3.4
	20	25 ± 0.22	125	0.88
oats	3	3.0 ± 0.060	100	2.0
	5	4.6 ± 0.080	92	1.7
	10	8.6 ± 0.11	86	1.3
	20	23 ± 0.67	115	2.9
rice	3	4.0 ± 0.17	133	4.2
	5	5.2 ± 0.07	104	1.3
	10	9.9 ± 0.67	99	6.8
	20	18 ± 1.4	90	7.8

^aEach assay was conducted three times on the same day.

^bThe assays were conducted on three different days.