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. 2020 Jun 17;3(3):198–210. doi: 10.1089/crispr.2019.0075

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© Jivan Khlghatyan and Jean-Martin Beaulieu 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — GSK3β of mPFC D2 neurons regulates anxiety-related behaviors. (A–D) Dark–light emergence test for Ctrl and Gsk3sKO in mPFC D2 mice. (A) Distance traveled in the light chamber (Ctrl: 357.9 ± 18.8 cm n = 18; Gsk3sKO in mPFC D2: 464.5 ± 29.1 cm n = 18). (B) Time spent in the light chamber (Ctrl: 64.6 ± 3.7 s n = 18; Gsk3sKO in mPFC D2: 85.9 ± 4.9 s n = 18). (C) Entries in the light chamber (Ctrl: 20.6 ± 1.5 n = 18; Gsk3sKO in mPFC D2: 28.00 ± 2.0 n = 18). (D) Total distance traveled (Ctrl: 1387 ± 41.3 cm n = 18; Gsk3sKO in mPFC D2: 1585 ± 61.6 cm n = 18). (E–G) Open field test for Ctrl and Gsk3sKO in mPFC D2 mice. (E) Center entries (Ctrl: 147 ± 9.5 n = 18; Gsk3sKO in mPFC D2: 162 ± 8.3 n = 18). (F) Activity in the center (Ctrl: 2293 ± 159.1 n = 18, Gsk3sKO in mPFC D2: 2425 ± 170.1 n = 18). (G) Activity in the border (Ctrl: 6923 ± 227 n = 18, Gsk3sKO in mPFC D2: 7111 ± 198 n = 18). (H) Elevated plus maze test for Ctrl and Gsk3sKO in mPFC D2 mice. Time in open arms (Ctrl: 29.5 ± 4.5 s n = 17; Gsk3sKO in mPFC D2: 45.8 ± 4.5 s n = 15). (I) Emotionality Z-score (Ctrl: −0.0000000033 ± 0.16 n = 18; Gsk3sKO in mPFC D2: −1 ± 0.25 n = 15). (J) Locomotion Z-score (Ctrl: −0.00000001 ± 0.19 n = 18; Gsk3sKO in mPFC D2: 0.6 ± 0.25 n = 15). (K) Total distance traveled every 5 min for Ctrl and Gsk3sKO in mPFC D2 mice after injection of saline at 30 min and methylphenidate at 90 min. Insert on the right shows total distance traveled between 30 and 90 min (Ctrl 1067 ± 121.2 n = 11; Gsk3sKO in mPFC D2 805.4 ± 105.8 n = 10) and 90 and 150 min (Ctrl 4384 ± 750.0 n = 11; Gsk3sKO in mPFC D2 3639 ± 590.8 n = 10). Error bars show SEM. *p < 0.05; **p < 0.01; ***p < 0.001; Student's t-test.