Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 17;3(3):164–176. doi: 10.1089/crispr.2020.0018

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Anna Buchman et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 1. — CasRx-mediated target transcript reduction in restricted tissue types using the binary Gal4/UAS system. (A) Representative genetic crossing schematic. (B) Inheritance rates of triple transheterozygous flies inheriting three transgenes (UASt-CasRx or UASt-dCasRx, gRNA^array, and Gal4-driver), corresponding to flies highlighted in the red box in panel A. Significant differences in inheritance between CasRx and dCasRx groups were observed in all three gene targets (gRNA^w, P = 0.00595; gRNA^N, P = 0.00402; gRNA^y, P = 0.02205). (C) Phenotypes of the triple transheterozygous flies. The white arrow identifies chitin pigment reduction in the thorax resulting from y targeting. Black and white fly with “X” represents a lethal phenotype with no live adults able to be scored or imaged. CasRx, Cas ribonuclease; gRNA^array, guide RNA array; gRNA^N, guide RNA targeting the Notch gene; gRNA^w, guide RNA targeting the white gene; gRNA^y, guide RNA targeting the yellow gene.