Fig. 3.

Robust CasRx-mediated reduction of GFP. (A) Representative bidirectional genetic crossing schematic. (B) Box plot of transheterozygote inheritance rates resulting from bidirectional crosses between Ubiq-CasRx (or Ubiq-dCasRx) and gRNA^GFP-OpIE2-GFP flies. (C) Images of F₁ larvae from paternal crosses demonstrating significant reduction in GFP expression for transheterozygous larvae expressing both Ubiq-CasRx and gRNA^GFP-OpIE2-GFP compared to control transheterozygotes expressing Ubiq-dCasRx and gRNA^GFP-OpIE2-GFP or without expressing a CasRx protein. (Left-right) Ubiq-CasRx/gRNA^GFP transheterozygous larvae, heterozygous gRNA^GFP larvae from Ubiq-CasRx cross, Ubiq-dCasRx/gRNA^GFP transheterozygous larvae, heterozygous gRNA^GFP larvae from Ubiq-dCasRx cross. CyO, ; dsRed, red fluorescent protein; GFP, green fluorescent protein; M, maternal inheritance of CasRx; P, paternal inheritance of CasRx; RFP, red fluorescent protein.