Fig 1. MES₅₅₀₀ is an effective immunosuppressor in vitro.

(A) Schematic diagrams of alternating current (AC), and different frequency pulse currents (low MES₅₅, middle MES₅₅₀ and high MES₅₅₀₀). (B-F) IL-2 and TNF-α mRNA levels were analyzed in Jurkat T cells treated with AC (B), MES₅₅ (C), MES₅₅₀ (D) or MES₅₅₀₀ (E, F), and stimulated with PMA/Io. (G-I) Primary mouse splenocytes treated with MES₅₅₀₀ and stimulated with PMA/Io were analyzed by qRT-PCR to detect the indicated genes. Data were normalized to the level of GAPDH mRNA (internal control). Data are presented as mean ± S.D. (n = 3 per group). *P < 0.05, ***P<0.001 assessed by Tukey-Kramer test. (n.s.; not significant). The data shown are representative of 2 or more independent experiments.