Fig 4. MES5500 shows anti-inflammatory effect via multiple pathways.
Primary mouse splenocytes (A) and Jurkat T cells (B-E) were treated with MES5500. Medium was changed and cells were stimulated with PMA/Io. (A-D) Nuclear and cytosolic fractions were isolated and subjected to immunoblotting using the indicated antibodies. (E) Total RNA was extracted to detect HO-1 mRNA level. Data were normalized to the level of GAPDH mRNA (internal control). Data are presented as mean ± S.D. (n = 3 per group), assessed by Tukey-Kramer test (n.s.; not significant). The data shown are representative of 2 or more independent experiments.