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. 2020 Jun 22;15(6):e0234867. doi: 10.1371/journal.pone.0234867

Fig 5. MES5500-treated cell-free culture media (MESCFM) shows immunosuppressive effect.

Fig 5

(A, B) IL-2 and TNF-α mRNA levels were analyzed in Jurkat T cells treated with MESCFM and stimulated with PMA/Io. Data were normalized to the level of GAPDH mRNA (internal control). Data are presented as mean ± S.D. (n = 3 per group) ***P<0.001 assessed by Tukey-Kramer test. (C-D) Immunoblotting of nuclear and cytosolic fractions from Jurkat T cells treated with MESCFM and/or PMA/Io. The data shown are representative of 2 or more independent experiments.