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. 2020 Jun 22;15(6):e0234867. doi: 10.1371/journal.pone.0234867

Table 1. Primers for RT-PCR.

Gene Primer sequence
Sense Antisense
Human IL-2 5’-GGATGCAACTCCTGTCTTGC-3’ 5’-GTGGCCTTCTTGGGCATGTA-3’
Human HO-1 5’-GGGAATTCTCTTGGCTGGCT-3’ 5’-GCTGCCACATTAGGGTGTCT-3’
Human IL-8 5’-CTGGCCGTGGCTCTCTTGT-3’ 5’-CCTTGGCAAAACTGCACCTT-3’
Human IL-1β 5’-ACGAATCTCCGACCACCACT-3’ 5’-CCATGGCCACAACAACTGAC-3’
Human IL-10 5’-AGAACCTGAAGACCCTCAGGC-3’ 5’-CCACGGCCTTGCTCTTGTT-3’
Human TGF-β 5’-ACCTGCCACAGATCCCCTAT-3’ 5’-CTCCCGGCAAAAGGTAGGAG-3’
Human TNF-α 5’-CAGCCTCTTCTCCTTCCTGA-3’ 5’-TGAGGTACAGACCCTCTGAT-3’
Mouse IL-2 5’-CCTGAGCAGGATGGAGAATTACA-3’ 5’-TCCAGAACATGCCGCAGAG-3’
Mouse IFN-γ 5’-CAACAACATAAGCGTCATTGAATCACAC-3’ 5’-GTTGACCTCAAACTTGGCAATACTC-3’
Mouse TNF-α 5’-CATCTTCTCAAAATTCGAGTGACAA-3’ 5’-TGGGAGTAGACAAGGTACAACCC-3’
Mouse IL-6 5’-GAGGATACCACTCCCAACAGACC-3’ 5’-AAGTGCATCATCGTTGTTCATACA-3’
Mouse IL-8 5’-TGTCAGTGCCTGCAGACCAT-3’ 5’-CCTCGCGACCATTCTTGAGT-3’
Mouse IL-10 5’-ATCGATTTCTCCCCTGTGAA-3’ 5’-TTCATGGCCTTGTAGACACCT-3’
Mouse TGF-β 5’-CACCTGCAAGACCATCGACAT-3’ 5’-GAGCCCTTAGTTTGGACAGCATCTG-3’