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. 2020 Jun 22;15(6):e0234638. doi: 10.1371/journal.pone.0234638

Fig 1. Valproic acid (VPA) and Stemreginin-1 (SR-1)-dependent effects on the growth of human hematopoietic stem cells (HSCs) in vitro.

Fig 1

Human HSCs were cultured in Stemline II Hematopoetic Stemcell Medium supplemented with 100 ng/ml FLT3, 100 ng/ml SCF, 100 ng/ml TPO and 50 ng/ml IL-3 (HSC medium). CD34+ cells were determined by flow cytometric analyses using an anti-CD34/CD45 mouse monoclonal antibody and 7AAD. (A) Fold amplification of all cells, CD34+ cells as well as percentage and absolute cell number of CD34+ cells after 14 days in vitro (DIV) are depicted for three different donors as their mean ± SD. HSCs were treated with 0.5 or 1 μM SR-1 (B), 0.5 or 1 mM VPA (C) or with 1 mM VPA in combination with 0.5 or 1 μM SR-1 (D) for 14 DIV are depicted for three different donors as their mean ± SD. *p<0.05.