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. 2020 May 22;46(2):709–717. doi: 10.3892/ijmm.2020.4615

Figure 2.

Figure 2

Overexpression of miR-29b promotes the osteogenic differentiation of hADSCs. hADSCs were transfected with agomir-29b, agomir NC, antagomir-29b and antagomir NC. At 48 h following transfection, cells were induced with differentiation medium. (A) RT-qPCR analysis was used to assess the expression levels of miR-29b 48 h after transfection. (B) Osteogenic differentiation was determined by staining with Alizarin Red S on day 21 post-induction. (C) Quantitative assessment of ALP activity by colorimetric alkaline phosphatase assay on day 21 post-induction. RT-qPCR analysis was used to assess the mRNA expression levels of the osteogenic differentiation markers (D) Runx2, (E) BSP, (F) OPN and osteocalcin (G) on day 21 post-induction. (H) Western blot analysis was used to assess the protein expression levels of the osteogenic differentiation markers (Runx2, BSP, OPN and osteocalcin). (I) Photograph morphology of hADSCs following Oil Red O, Alizarin Red S or Alcian blue staining after 21 days of culture in normal, adipogenic, osteogenic or chondrogenic differentiation medium. Magnification, x200. Data were represented as the means ± SD of 3 independent experiments. **P<0.01 vs. agomir NC group; ##P<0.01 vs. antagomir NC group. hADSC, human adipose derived mesenchymal stem cell; Runx2, runt-related transcription factor 2; BSP, bone sialoprotein; OPN, osteopontin.