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. 2020 May 22;46(2):709–717. doi: 10.3892/ijmm.2020.4615

Figure 5.

Figure 5

PTEN reverses the promoting effects of miR-29b on the osteogenic differentiation of hADSCs. hADSCs were co-transfected with agomir-29b and pcDNA-PTEN, following induction of osteogenic differentiation. (A) The expression of PTEN protein was measured in hADSCs when the pcDNA-PTEN plasmid was transfected by western blot analysis. (B) Western blot analysis was used to assess the protein expression levels of PTEN in hADSCs following transfection with agomir-29b and pcDNA-PTEN. (C) Osteogenic differentiation was determined by staining with Alizarin Red S staining on day 21 post-induction. Shown is a representative example of 3 independent experiments. (D) Quantitative assessment of ALP activity by colorimetric alkaline phosphatase assay on day 21 post-induction. RT-qPCR analysis was used to assess the mRNA expression levels of the osteogenic differentiation markers (E) Runx2, (F) BSP, (G) OPN and osteocalcin (H) on day 21 post-induction. Data represent the means ± SD of 3 independent experiments. *P<0.05, **P<0.01 vs. agomir NC group, ##P<0.01 vs. agomir-29b + pcDNA-vector group. PTEN, phosphatase and tensin homolog; hADSC, human adipose derived mesenchymal stem cell.