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. 2020 Jun 4;46(2):663–674. doi: 10.3892/ijmm.2020.4631

Figure 5.

Figure 5

Effect of TRAP1 on MPTP opening. (A) MPTP opening was detected using a flow cytometer. The fluorescence intensity was negatively associated with MPTP opening. (B) Quantitative analysis of the results shown in (A). **P<0.01. ov-Con, H9C2 cells transfected with vector; ov-TRAP1, H9C2 cells overexpressing TRAP1; sh-Con, H9C2 cells transfected with scrambled siRNA; sh-TRAP1, H9C2 cells transfected with TRAP1-siRNA; ov-TRAP1 + Atr, H9C2 cells overexpressing TRAP1 after adding Atr (10 µM) to the culture medium; sh-TRAP1 + CysA, H9C2 cells transfected with TRAP1-siRNA in the presence of CysA (10 µM) in the culture medium; TRAP1, tumor necrosis factor receptor-associated protein 1.