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Yeast-one-hybrid (Y1H) screening and EMSA analysis. (A) Schematic diagram of the bait fragments (F and G) used to construct the reporter vectors in the Y1H assay. (B) EMSA analysis with in vitro translated OsbZIP48, OsbZIP49, and, as reference, AtbZIP19 and AtbZIP23. Probe acronyms 2Z and 3Z correspond to two or three ZDRE (ATGTCGACAY) tandem repeats, respectively, and 3mZ corresponds to three tandem repeats of a ZDRE mutated version (ATGTAGACAY).
