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. 2020 Mar 5;71(12):3664–3677. doi: 10.1093/jxb/eraa115

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Gene expression analysis of 4-week-old hydroponically grown rice plants in response to Zn supply. Shoot and root transcript levels of (A) OsbZIP48, (B) OsbZIP49, (C) OsbZIP50, (D) OsZIP2, (E) OsZIP4, (F) OsZIP8, and (G) OsZIP10, grown with control or –Zn nutrient solution. Bars represent mean fold change in the transcript level of three biological replicates ±SE. Statistically significant differences between control and –Zn conditions were determined by Student t-test (**P<0.01, ***P<0.001; ns indicates not significant). (H) Analysis of the number and position of detected ZDRE motifs in the promoter of each gene. ^aPosition of the first nucleotide of the ZDRE motif in relation to the start codon. ^bMismatches from the ZDRE consensus sequence (RTGTCGACAY).