Table 2. Melting Temperatures of p53-Y220X Mutant DBDs.

codon 220 mutationa	Tm (°C)a	ΔTm (°C)b
WT residue	51.5 ± 0.1
Y220H	45.1 ± 0.1	–6.4
Y220C	43.7 ± 0.1	–7.8
Y220N	39.9 ± 0.2	–11.6
Y220S	39.4 ± 0.2	–12.1

^aMelting temperatures of Y220X mutants were measured in the framework of a stabilized quadruple mutant DBD (M133L/V203A/N239Y/N268D). The T_m of the wild-type DBD is ∼45 °C.^32,57 Quadruplicate measurements, mean ± SEM is given.

^bΔT_m = T_m (mutant) – T_m (wild type).