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. 2020 Jan 17;11(3):1141–1147. doi: 10.1021/acs.jpclett.9b03208

Figure 4.

Figure 4

DEER results for PpiB K25C/E153C-BrPy-DO3A-Gd(III). (a) W-band ED-EPR spectra in HeLa cells incubated 4 h (red) and 12 h (blue) after electroporation. The ED-EPR traces were normalized to the intensity of endogenous Mn(II). (b) Primary DEER traces and background function (gray) of the 4 h (red) and 12 h (blue) in-cell samples compared with the solution sample (50 μM, black). (c) DEER traces shown in panel b after background removal and the fitting traces obtained with Tikhonov regularization using DeerAnalysis (gray). (d) Distance distributions.