Table 1. Estimated in-Cell Spin Concentration for Samples Studied in This Work.
in-cell
EPR spin concentration (μM)a |
||
---|---|---|
4 h | 12 h | |
PpiB-CT02MA | 31.8 ± 6.7 | 11.7 ± 3.1 |
PpiB-BrPy-DO3A-Gd(III) | 46.1 ± 12.5 | 29.0 ± 8.3 |
GB1-CT02MA | 35.4 ± 7.4 | 12.5 ± 3.2 |
Protein concentration is about half that of the spin concentration if we consider 100% labeling efficiency.