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. 2020 Jun 22;551:84–92. doi: 10.1016/j.virol.2020.05.014

Fig. 1.

Fig. 1

Bat MRVs have wide cell tropism. MdKi, PaKi, HpLuT, MDCK, A549, LLC-MK2, FK and Hela cells were infected with bat MRV WIV2, WIV3 and WIV7 (MOI = 1). Immunofluorescence assay (Panel 1–4) was performed at 24 hpi. Bat MRV antigens were stained with rabbit anti-WIV3 polyclonal antibody as the primary antibody followed by FITC-labelled mouse anti-rabbit IgG and coloured in green. Cell nuclei were stained with DAPI and shown in blue. Scale bar is 50 μm. Multi-cycle supernatants were taken at 0, 12, 24, 48 and 72 hpi. Viral loads were determined by RT-qPCR (panel 5). Error bar indicates the standard error. * represent WIV-2 group compared with WIV-7 group, # represent WIV-3 group compared with WIV-7 group, ※ represent WIV-2 group compared with WIV-3 group. *P < 0.05, **P < 0.01, ***P < 0.001.