Fig. 4.

Bat MRVs infection cause severe pneumonia in BALB/c mice. Left lung lobe of BALB/c mice at 14 dpi were subjected to pathological exam by H&E staining and IHC assay, images were taken from the base of lungs. A and E, mock-infected mice examined by H&E staining and IHC, respectively. B-D, mice infected by WIV2, WIV3 and WIV7, respectively, was examined by H&E staining. The tested tissues showed severe pneumonia with distinct alveolar thickening, reduction of bronchioles and alveoli (black box) and lymphocytic infiltration (black arrow). Scale bar is 200 μm. For IHC assay, rabbit anti-WIV3 polyclonal antibody was used as the primary antibody and HRP-labelled goat anti-rabbit IgG was used as the secondary antibody. The positive area is brownish yellow and can be observed in WIV2- (F), WIV3- (G) and WIV7 (H)-infected lungs. Scale bar is 100 μm.