Figure 2. Fbxo7 triggers mitochondrial injury.

(A and C) BEAS-2B cells were nucleofected with control or Fbxo7 plasmid for 48 hours or shRNAs for 72 hours. Cells were treated with 50 μM CCCP for 5 minutes where indicated, and then stained with JC1 (2 μM) for additional 20 minutes for confocal microscopic analysis. Scale bars: 10 µm. (B) BEAS-2B cells were nucleofected with control or Fbxo7 plasmid for 48 hours and then were treated with or without CCCP (20 μM) for 1 hour. The cells were stained with MitoSense Red and annexin V for flow cytometry analysis.