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. 2020 Jun 4;5(11):e137393. doi: 10.1172/jci.insight.137393

Figure 7. NE- and 48/80-evoked thermal hyperalgesia is not mediated via PAR2+ sensory neurons.

Figure 7

Mice were injected with PAR2 agonists and then latency to paw withdrawal was measured via the Hargreaves test 1, 3, 5, 24, and 48 hours after hind paw injection. Baseline (BL) measures were obtained before administering 2AT, 48/80, or NE. When compared with F2rl1floxPirt+/+ mice, F2rl1floxPirtCre mice showed decreased thermal hyperalgesia in response to only 2AT (30 pmol) (A) but not to 48/80 (6.5 nmol) (B) or NE (10 units) (C). Effect size is determined by calculating the cumulative difference between the baseline value and the value for each time point. *P < 0.05 compared with F2rl1floxPirt+/+ or F2rl1floxPirtCre groups. #P < 0.05 compared with baseline measures. n = 4 for F2rl1floxPirt+/+ and F2rl1floxPirtCre groups treated with 2AT and 48/80, and n = 7 for F2rl1floxPirt+/+ and F2rl1floxPirtCre groups treated with NE. Data are expressed as mean ± SEM. Two-way ANOVA with Holm-Šidák and Dunnett’s multiple comparisons #P < 0.05, ##P < 0.01, and ###P < 0.001. Unpaired t test *P < 0.05.