Figure 8. Effects of 48/80 and NE on paw temperature are sensory neuron PAR2-mediated.

Mice were injected with PAR2 agonists, and then hind paw temperatures were measured via FLIR imaging 1, 3, 5, 24, and 48 hours after hind paw injection. Baseline measures were obtained before administering 2AT, 48/80, or NE. 2AT (30 pmol) (A) did not cause an increase in paw temperature in either genotype. Unlike the F2rl1^floxPirt^+/+ mice, F2rl1^floxPirt^Cre mice did not show an increase in paw temperature in response to 48/80 (6.5 nmol) (B) and NE (10 units) (C). Effect size is determined by calculating the cumulative difference between the value for each time point and the baseline value. *P < 0.05 compared with F2rl1^floxPirt^+/+ or F2rl1^floxPirt^Cre groups. ^#P < 0.05 compared with baseline measures. n = 4 for F2rl1^floxPirt^+/+ and F2rl1^floxPirt^Cre groups treated with 2AT and 48/80, and n = 7 for F2rl1^floxPirt^+/+ and F2rl1^floxPirt^Cre groups treated with NE. Data are expressed as mean ± SEM. Two-way ANOVA used for temperature, with Holm-Šidák and Dunnett’s multiple comparisons: *P < 0.05, and **P < 0.01; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001. Unpaired t test used for effect size: *P < 0.05, and **P < 0.01. Asterisks denote significant differences between genotypes. Pound signs denote significant differences versus BL as a function of time.