Figure 6. Suppression of adult hepatic mononuclear cells by neonatal hepatic mononuclear cells.

Adult hepatic mononuclear cells (MNCs) can produce TNF-α upon anti-CD3 stimulation; therefore, they were used as responder cells. Neonatal liver harvested at 24 hours of life, or 3 days after antibody depletion followed by RRV infection, was used as erythroblast pool to function as effector cells. TNF-α levels were measured in supernatant 72 hours after coculture. “+” denotes presence. “–” denotes absence. One-way ANOVA test was performed. *P < 0.05, ***P < 0.001. n = 3/group.