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. 2020 Mar 5;9(3):623–633. doi: 10.1021/acssynbio.9b00468

Figure 2.

Figure 2

Initial assessment of using Y. lipolytica to produce 2-PE from l-phenylalanine via the Ehrlich pathway. (a–c) 2-PE and phenylacetate titer, time profiles of cell growth, and 2-PE yield of adding different concentrations l-phenylalanine (0, 2, 4, 6, 8, and 10 g/L). (d–f) 2-PE titer, yield, and cell growth of respective overexpressing genes ylARO8, ylARO9, and ylARO10. All experiments were performed in triplicate and error bars show standard deviation (SD). The * indicates p < 0.01, and ** indicates p < 0.05 (two-tailed test).