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. 2020 Mar 5;9(3):623–633. doi: 10.1021/acssynbio.9b00468

Figure 3.

Figure 3

Characterization of upstream module of the Ehrlich pathway: l-phenylalanine specific permeases and l-phe: 2-oxoglutarate transaminase. (a) Flow-chart of shake flask cultivations of engineered strains. (b–d) 2-PE titers, cell growth, and 2-PE yield of respective overexpressing genes ylARO10 and ylARO8, ylARO10 and ylARO9, and ylARO10 and C18645. (e,f) 2-PE titers and 96h residual glucose of screening l-phenylalanine specific permeases with CSM fermentation medium in shake cultivations. All experiments were performed in triplicate and error bars show standard deviation (SD). The * indicates p < 0.01, and ** indicates p < 0.05 (two-tailed test).