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. 2020 Mar 5;9(3):623–633. doi: 10.1021/acssynbio.9b00468

Figure 5.

Figure 5

Blocking competitive pathways and integration of the Ehrlich pathway in 26s rDNA sites to improve 2-PE production. (a–c) Cell growth, 2-PE titers, phenylacetate titers, and 2-PE yield of blocking competitive pathways. (d) Screening of the productive strains based on 24-well deep plate cultivations. (e,f) 2-PE titer and yield by further validating productive strains with integration of the Ehrlich pathway in flask cultivations. 1, strain po1fk4–1; 2, strain po1fk4–2; 3, strain po1fk4–3; 4, strain po1fk4–4; 5, strain po1fk4–5; 6, strain po1fk4–6; 7, strain po1fk4–7; 8, strain po1fk4–8; 9, strain po1fk4–9; 10, strain po1fk4–10; 11, strain po1fk4–11; 12, strain po1fk4–12. All experiments were performed in triplicate and error bars show standard deviation (SD).