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Overcoming cellular compartmentalization of 2-oxoglutarate (aKG) to increase aKG availability. (a) Construction of cytosolic source for 2-oxoglutarate synthesis by coupling bacterial aconitase with the overflowed citrate from Krebs cycle. (b) Citrate titers of strain po1fk4–2. (c–e) 2-PE titers, cell growth and yield of strains po1fk4P1, po1fk4P2, po1fk4P3, and po1fk4P4. All experiments were performed in triplicate and error bars show standard deviation (SD).
