Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX:000664 | |
| Genetic reagent (Mus musculus) |
Tg(Fev-flpe)1Dym
Referred to as Pet1-Flpe |
PMID:18344997 | RRID:MGI:5004974 | |
| Genetic reagent (Mus musculus) |
En1tm2(cre)Wrst
Referred to as En1-cre |
PMID:10837030 | RRID:IMSR_JAX:007916 | |
| Genetic reagent (Mus musculus) |
Tg(Slc6a4-cre)ET33Gsat
Referred to as Slc6a4-cre |
PMID:17855595 | RRID:MGI:3836639 | |
| Genetic reagent (Mus musculus) |
Npy2rtm1.1(cre)Lbrl
Referred to as Npy2r-cre |
PMID:25892222 | RRID:IMSR_JAX:029285 | Lab of Steve Liberles |
| Genetic reagent (Mus musculus) |
P2ry1tm1.1(cre)Lbrl
Referred to as P2ry1-cre |
PMID:25892222 | RRID:IMSR_JAX:029284 | Lab of Steve Liberles |
| Genetic reagent (Mus musculus) |
Tg(Crh-cre)KN282Gsat/Mmucd
Referred to as Crh-cre |
RRID:MMRRC_030850-UCD | ||
| Genetic reagent (Mus musculus) |
Gad2tm2(cre)Zjh
Referred to as Gad2-cre |
PMID:21943598 | RRID:IMSR_JAX:028867 | |
| Genetic reagent (Mus musculus) |
Slc17a8tm1.1(cre)Hz
Referred to asSlc17a8- cre |
MGI: J:146821 | RRID:IMSR_JAX:028534 | |
| Genetic reagent (Mus musculus) |
Gt(ROSA)26Sortm8(CAG-mCherry,-EGFP)Dym
Referred to as RC-FrePe |
PMID:22151329 | RRID:IMSR_JAX:029486 | |
| Genetic reagent (Mus musculus) |
Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze
Referred to as RC-Ai65 |
PMID:25741722 | RRID:IMSR_JAX:021875 | |
| Genetic reagent (Mus musculus) |
GT(ROSA)26Sortm3.2(Cag-EGFP,CHRM3*/mCherry/Htr2a)Pjen
Referred to as RC-FL-hM3Dq |
PMID:27264177 | RRID:IMSR_JAX:026942 | |
| Recombinant DNA reagent | pENN.AAV.hSyn.Cre.WPRE.hGH (AAVrg Viral prep) | Addgene | Cat# 10553-AAVrg RRID:Addgene_105553 |
|
| Antibody | anti-GFP (chicken polyclonal) | Aves Labs | Aves Labs Cat# GFP-1020, RRID:AB_10000240 | IHC (1:3000) |
| Antibody | anti-DsRed (rabbit polyclonal) | Takara Bio | Takara Bio Cat# 632496, RRID:AB_10013483 | IHC (1:1000) |
| Antibody | anti-PAX5 (goat polyclonal) | Santa Cruz | Santa Cruz Biotechnology Cat# sc-1974, RRID:AB_2159678 | IHC (1:1000) |
| Antibody | anti-SATB2 (guinea pig polyclonal) | Synaptic Systems | Synaptic Systems Cat# 327 004, RRID:AB_2620070 | IHC (1:1000) |
| Antibody | anti-COUP-TFII (anti-NR2F2, mouse monoclonal) | Perseus Proteomics | Perseus Proteomics Cat# PP-H7147-00, RRID:AB_2314222 | IHC (1:1000) |
| Antibody | anti-VGLUT3 (guinea pig polyclonal) | Synaptic Systems | Synaptic Systems Cat# 135 204, RRID:AB_2619825 | IHC (1:500) |
| Antibody | anti-RFP (rat monoclonal) | Chromotek | ChromoTek Cat# 5f8-100, RRID:AB_2336064 | IHC (1:500) |
| Antibody | anti-DCX (goat polyclonal) | Santa Cruz | Santa Cruz Biotechnology Cat# sc-8066, RRID:AB_2088494 | IHC (1:1000) |
| Antibody | anti-Ki67 (rat monoclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# 14-5698-80, RRID:AB_10853185 | IHC (1:1000) |
| Antibody | anti-TPH2 (rabbit polyclonal) | Novus Biologicals | Novus Cat# NB100-74555, RRID:AB_1049988 | IHC (1:1000) |
| Antibody | Alexa Fluor 488 (donkey anti-chicken) | Jackson | Jackson ImmunoResearch Labs Cat# 703-545-155, RRID:AB_2340375 | IHC (1:500) |
| Antibody | Alexa Fluor 546 (donkey anti-rabbit) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A10040, RRID:AB_2534016 | IHC (1:500) |
| Antibody | Alexa Fluor 647 (donkey anti-goat) | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A-21447, RRID:AB_2535864 | IHC (1:500) |
| Antibody | Alexa Fluor 647 (donkey anti-mouse) | Jackson | Jackson ImmunoResearch Labs Cat# 715-605-151, RRID:AB_2340863 | IHC (1:500) |
| Antibody | Alexa Fluor 647 (donkey anti-rabbit) | ThermoFisher Scientific | Thermo Fisher Scientific Cat# A-31573, RRID:AB_2536183 | IHC (1:500) |
| Antibody | Cy5 (donkey anti-guinea pig) | Jackson | Jackson ImmunoResearch Labs Cat# 706-175-148, RRID:AB_2340462 | IHC (1:500) |
| Commercial assay or kit | Tph2-C2 probe | ACDBio | ACDBio:318691-C2 | |
| Commercial assay or kit | Chromium Single Cell v3 Reagent Kits | 10X Genomics | 10X Genomics:1000092/1000074 | |
| Commercial assay or kit | SMARTseq V4 Ultra Low Input RNA Kit | Takara Bio | Takara Bio:634890 | |
| Commercial assay or kit | Nextera XT DNA Library Preparation Kit | Illumina | Illumina: FC-131–1024 | |
| Commercial assay or kit | RNAscope Fluorescent Multiplex Reagent Kit | ACDBio | ACDBio:320850 | |
| Software, algorithm | R (Version 3.5.3, 3.6.3) | R Project for Statistical Computing | R Project for Statistical Computing, RRID:SCR_001905 | https://cran.r-project.org/ |
| Software, algorithm | Seurat (Versions 3.0.2, 3.1.1, 3.1.4) | PMID:29608179 | Seurat, RRID:SCR_016341 | https://satijalab.org/seurat/ |
| Software, algorithm | Fiji (Version 2.0.0-rc-69/1.52 p) | PMID:22743772 | Fiji, RRID:SCR_002285 | https://imagej.net/Fiji |