Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene Saccharomyces cerevisiae | Yeast Genome | UCSC | SacCer3 | |
| Strain, strain background (Escherichia coli) | BL21-CodonPlus (DE3)RIL | Agilent | Cat# 230245 | |
| Antibody | Anti-Sth1 (Rabbit polyclonal) |
Cairns et al., 1996 | (1:1000) | |
| Antibody | Anti-Rsc3 (Rabbit polyclonal) |
Angus-Hill et al., 2001 | (1:1000) | |
| Antibody | Anti-Rsc30 (Rabbit polyclonal) |
Angus-Hill et al., 2001 | (1:1000) | |
| Antibody | Anti-Rsc2 (Rabbit polyclonal) |
Kasten et al., 2004 | (1:1000) | |
| Antibody | Anti-Rsc4 (Rabbit polyclonal) |
Kasten et al., 2004 | (1:1000) | |
| Antibody | Anti-HA (mouse monoclonal) |
Cairns et al., 1999 | (1:1000) | |
| Antibody | Anti-Myc (mouse monoclonal) |
Abcam | Cat# ab56 RRID:AB_304876 |
(1:1000) |
| Antibody | Anti-V5 (mouse monoclonal) |
Thermo Scientific | Cat# R960-25 RRID:AB_2556564 |
(1:1000) |
| Peptide, recombinant protein | 8XHIS.HMO1 | This paper, Figure 4 | purified from E. coli BL21-CodonPlus(DE3)-RIL cells | |
| Peptide, recombinant protein | Histone H2A, Oregon green, yeast octamers |
Xin et al., 2009 | ||
| Peptide, recombinant protein | Histone H3 R40A | This paper, Figure 3 | purified from E. coli BL21-CodonPlus(DE3)-RIL cells | |
| Peptide, recombinant protein | AcTEV protease | Thermo Scientific | Cat# 12575015 | |
| Peptide, recombinant protein | Micrococcal Nuclease | USB | Cat# 70196Y | |
| Peptide, recombinant protein | Exonuclease III | New England Biolabs | Cat# M0206 | |
| Peptide, recombinant protein | S1 nuclease | Thermo Scientific | Cat# 18001–016 |
|
| Peptide, recombinant protein | Klenow fragment | New England Biolabs | Cat# M0212L | |
| Commercial assay or kit | NEBNext ChIP-Seq MasterMix Set | New England Biolabs | Cat# E6240L | |
| Commercial assay or kit | RNEasy | Qiagen | Cat# 74106 | |
| Commercial assay or kit | RiboPure RNA purification kit, Yeast | Thermo Scientific | Cat# AM1926 | |
| Commercial assay or kit | TruSeq Stranded Total RNA Library Prep Kit with Ribo Zero Gold | Illumina | Cat# RS-122–2301 | |
| Commercial assay or kit | BioRad protein assay reagent | BioRad | Cat# 500–0006 |
|
| Commercial assay or kit | Minelute PCR purification kit | Qiagen | Cat#28006 | |
| Chemical compound, drug | 5-Fluoroorotic Acid (5FOA) | Toronto Research Chemicals | Cat# F59500 | |
| Chemical compound, drug | Mycophenolic Acid (MPA) | Calbiochem | Cat# 475913 | |
| Software, algorithm | Prism | GraphPad Software Inc | RRID:SCR_002798 | Version 8.0.2 |
| Software, algorithm | DESeq2 | Bioconductor | RRID:SCR_015687 | Version 1.21 |
| Software, algorithm | STAR | Dobin et al., 2013 | RRID:SCR_015899 | Version 2.5.4 |
| Software, algorithm | R | R Development Core Team, 2018 | RRID:SCR_001905 | |
| Software, algorithm | pHeatmap | Kolde, 2019 | RRID:SCR_016418 | |
| Software, algorithm | Novoalign | Novocraft | RRID:SCR_014818 | Version 3.8.2 |
| Software, algorithm | UMI Scripts | Huntsman Cancer Institute | See Materials and methods for github link | |
| Software, algorithm | BioToolBox packages | TJ Parnell, Huntsman Cancer Institute | See Materials and methods for github link | |
| Software, algorithm | ImageQuant TL | GE Healthcare | RRID:SCR_018374 | |
| Other | IgG Sepharose Fast Flow | GE Healthcare | Cat# GE 17-0969-01 |
|
| Other | Calmodulin Affinity Resin | Agilent | Cat# 214303 |
|
| Other | Nickel-NTA Agarose Beads | Qiagen | Cat# 30230 |
|
| Other | Slide-A-Lyzer mini dialysis units | Thermo Scientific | Cat# 69560 |
|
| Other | Dynabeads Pan Mouse IgG | Thermo Scientific | Cat#11041 |