Table 1. Summary of Histone H3-H4 screen with rsc1∆ and rsc2∆.
Library of TRP1-marked plasmids containing H3-H4 residues mutated to alanine were transformed into h3-h4Δ [H3-H4.URA3] (YBC1939), rsc1Δ h3-h4Δ [H3-H4.URA3] (YBC2090) or rsc2Δ h3-h4Δ [H3-H4.URA3] (YBC3040), and spotted to SC-TRP, or SC-TRP + 5FOA to force the loss of the WT histone plasmid and test for synthetic lethality. Histone mutations that were lethal on their own in WT RSC are shaded grey, lethal in combination with rsc1Δ are in bold and highlighted yellow, lethal with rsc2Δ are italicized and highlighted in blue, and residues that were lethal with both rsc1Δ and rsc2Δ are highlighted in green.
| H3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | R2A | T11A | L20A | T32A | R40A | R49A | S57A | L65A | E73A | L82A | L92A | V101A |
| B | T3A | G12A | S22A | G33A | Y41A | E50A | T58A | P66A | I74A | R83A | Q93A | S102A |
| C | K4A | G13A | K23A | G34A | K42A | I51A | E59A | F67A | Q76A | F84A | E94A | L103A |
| D | Q5A | K14A | R26A | V35A | P43A | R52A | L60A | Q68A | D77A | Q85A | S95A | F104A |
| E | T6A | P16A | K27A | K36A | G44A | R53A | L61A | R69A | F78A | S86A | V96A | E105A |
| F | R8A | R17A | S28A | K37A | T45A | F54A | I62A | L70A | K79A | S87A | E97A | D106A |
| G | K9A | K18A | P30A | P38A | V46A | Q55A | R63A | V71A | T80A | I89A | Y99A | T107A |
| H | S10A | Q19A | S31A | H39A | L48A | K56A | K64A | R72A | D81A | G90A | L100A | N108A |
| H3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | L109A | Q120A | R129A | |||||||||
| B | I112A | K121A | L130A | |||||||||
| C | H113A | K122A | R131A | |||||||||
| D | K115A | D123A | G132A | |||||||||
| E | R116A | I124A | E133A | |||||||||
| F | V117A | K125A | R134A | |||||||||
| G | T118A | L126A | S135A | |||||||||
| H | I119A | R128A | ||||||||||
| H4 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | S1A | G9A | H18A | I26A | R35A | K44A | E52A | F61A | S69A | R78A | V86A | R95A |
| B | G2A | L10A | R19A | Q27A | R36A | R45A | E53A | L62A | V70A | K79A | V87A | T96A |
| C | R3A | G11A | K20A | G28A | L37A | I46A | V54A | E63A | T71A | T80A | Y88A | L97A |
| D | G4A | K12A | I21A | I29A | R39A | S47A | R55A | S64A | Y72A | V81A | L90A | Y98A |
| E | K5A | G13A | L22A | T30A | R40A | G48A | V57A | V65A | T73A | T82A | K91A | G99A |
| F | G6A | G14A | R23A | K31A | G41A | L49A | L58A | I66A | E74A | S83A | R92A | F100A |
| G | G7A | K16A | D24A | P32A | G42A | I50A | K59A | R67A | H75A | L84A | Q93A | G101A |
| H | K8A | R17A | N25A | I34A | V43A | Y51A | S60A | D68A | K77A | D85A | G94A | G102A |
| Lethal w/rsc1∆ | Lethal w/rsc2∆ | Lethal w/rsc1∆ and rsc2∆ | Lethal w/WT | |||||||||