Table 2. *DNA end to end FRET measurements on mononucleosomes containing H3 αN helix mutations from Ferreira et al., 2007 with the phenotype when combined with RSC, rsc1∆, or rsc2∆.
| Mutation | * FRET % | Phenotype |
|---|---|---|
| WT | 100 ± 6 | No phenotype |
| R40A | 71 ± 7 | Lethal w/rsc1∆ |
| Y41A | 72 ± 7 | Lethal w/WT |
| K42A | 54 ± 3 | Lethal w/rsc1∆ |
| P43A | 91 ± 3 | No phenotype |
| G44A | 68 ± 9 | Lethal w/rsc1∆ |
| T45A | 52 ± 5 | Lethal w/WT |
| V46A | 89 ± 5 | Lethal w/rsc1∆ |
| L48A | 86 ± 4 | Lethal w/WT |
| R49A | 67 ± 3 | Lethal w/rsc1∆ |
| E50A | 98 ± 6 | No phenotype |
| I51A | 81 ± 13 | Lethal w/WT |
| R52A | 78 ± 3 | Lethal w/rsc1∆ |
| R53A | 80 ± 4 | No phenotype |
| F54A | 96 ± 3 | Lethal w/WT |
| Q55A | 69 ± 11 | Lethal w/WT |
| K56A | 95 ± 4 | No phenotype |
| S57A | 102 ± 5 | No phenotype |
| K56Q | 82 ± 2 | Lethal w/rsc1∆ |