Table 2. Variants observed in each library group.

			37°C*		25°C*
Group	Number of positions	Total possible variants	Both replicates†	At least one replicate‡	Both replicates	At least one replicate
1	39	819	808	811	808	812
2	39	819	812	813	811	814
3	39	819	803	807	802	807
4	39	819	802	813	809	812
5	39	819	801	812	804	811
6	39	819	775	793	789	796
7	33	693	682	686	678	690
Total	267	5607	5483	5535	5501	5542
% coverage			97.8%	98.7%	98.1%	98.8%

*Non-selected libraries were grown, sequenced and filtered separately at 37°C and 25°C.

†The observed number of variants that passed noise filtering in both sequencing replicates of the non-selected library.

‡The observed number of variants that passed noise filtering in at least one sequencing replicate of the non-selected library.