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. Author manuscript; available in PMC: 2020 Jun 22.
Published in final edited form as: Exp Cell Res. 2020 Mar 21;390(2):111961. doi: 10.1016/j.yexcr.2020.111961

Fig. 2. Restoring actin Nt-acetylation decreases Golgi fragmentation in NAA80 KO cells.

Fig. 2

A. HAP1 NAA80 KO cells were transfected with either EGFP-control vector, NAA80-EGFP or catalytically dead NAA80mut-EGFP, fixed 24 h post transfection and IF stained for GM130. Representative confocal STED images from three independent experiments are shown; scale bar = 20 μm. B. Quantification of Golgi fragmentation in the transfected NAA80 KO cells shown in A. The mean ± sd of three independent experiments (each with n ≥ 200 cells) is shown; *p ≤ 0.05, one-way ANOVA with Tukey’s multiple comparison test. C. Western blot analysis of EGFP, actin Nt-acetylation (Ac-β-actin), pan actin and NAA80 in whole cell lysates of transfected NAA80 KO cells. Cells were transfected as in A and harvested 24 h post transfection. Representative blots from two independent experiments are shown.