Extended Data Figure 7. MMP activity is indispensable for proper embryo growth.

A) Stills from time lapse video of representative control(n=5 embryos) and MMP inhibitors-treated(n=4 embryos) (100uM NSC405020 + 20uM Prinomastat) embryos cultured from E5.75 for 18 hours. Red arrowheads show cell death initiation after growth restriction in the absence of MMPs activity. B) Control (n=20) and MMP inhibitors-treated(n=18) embryos (18 hours). C) Quantification of embryo length (18 hours of culture). n=20 control and 18 MMP inhibitors-treated embryos; Two sided unpaired student’s t-test; ****p<0.0001; mean±SEM. D) Representative examples of control (n=7) and MMP inhibitors treated embryos(n=6) stained with the apoptotic marker c-casp. E) Quantification of apoptotic index (number of apoptotic cells/ total cell number). n=7 control and 6 MMP inhibitors treated embryos.Two sided unpaired student’s t-test; ***p=0.0002, mean±SEM F) Representative examples of control(n=10) and MMP inhibitors treated(n=10) embryos stained with the mitotic marker phospho-Histone H3(Ser 10). G) Quantification of mitotic index (number of mitotic cells/ total cell number). n=10 control and 10 MMP inhibitors treated embryos. Two sided unpaired student’s t-test, mean±SEM. Scale bars=20um.