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. 2020 Jun 18;13:5795–5805. doi: 10.2147/OTT.S257512

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© 2020 Yang et al.

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Knockdown of B7-H6 inhibited NHL cell proliferation, migration and invasion. (A and B) Transfection with recombinant lentiviral shRNA (shB7-H6) was performed to establish stable B7-H6 knockdown Jurkat and Raji cells. The expression of B7-H6 in cells transfected with shB7-H6 compared to that of the corresponding control-vector groups (shCtrl) was evaluated by RT-qPCR (A) and Western blot (B). (C) The effects of B7-H6 on cell proliferation were analyzed by CCK-8 assays in Jurkat and Raji cells at 0h, 24h, 48h and 72h. (D and E) The effects of B7-H6 on cell migration and invasion were assessed by Transwell assays in Jurkat and Raji cells. *P<0.05, **P<0.01.