Figure 2.

CFTR inhibits NF-κB signaling pathway. CFTR overexpression, knockout and contrast cells were constructed in HeLa lines. (A) Green fluorescence in cell lines that transfected stable CFTR expression successfully. (B) CFTR protein was measured by Western blot analysis (~170KD). β-Actin was used as an internal control (~43KD). (C) The protein expression of NF-κB p65 and IκBα was tested in four cell lines. (D) Expression of NF-κB p65 under the action of CFTR activator and inhibitor in HeLa cells.