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Examples of RBF biosensors for RNA sensing. (A) Split-binding domain strategy was applied on Spinach and SRB-2 aptamer to generate biosensors for miRNA [52•,27•]. (B) Destabilizing the fluorogenic aptamer by shortening a stem or splitting led to functional biosensors for mRNA [28,53]. (C) For sensitive RNA detection, the catalytic hairpin assembly was used to amplify the signal triggered by target RNA [55].
