Table 1.

Characteristics of the custom-made targeted gene panel

Characteristics	Panel I
Type of sample	FFPE-DNA
Amplicon length, bp	125–175
Amplicons designed	399 (X 2)
Common genes included (pituitary adenoma and DTC)	1. AIP (NM_003977): exon 1–6;
	2. BRAF (NM_004333): exon 15;
	3. CDKN1B (NM_ 004064): exon 1–2
	4. GNAS (NM_016592): exon 1–13
	5. GPR101 (NM_054021): exon 1;
	6. HRAS (NM_005343): exon 2–6;
	7. KRAS (NM_004985): exon 2–5;
	8. MEN1 (NM_000244): exon 1–10;
	9. NRAS (NM_002524): exon 3;
	10. PIK3CA (NM_006218): exon 2–21;
	11. PRKACB (NM_207578): exon 1–10;
	12. PRKAR1A (NM_212471): exon 2–11;
	13. RET (NM_020975): exon 2–20;
	14. SDHA (NM_004168): exon 2–15;
	15. SDHAF2 (NM_017841): exon 1– 4;
	16. SDHB (NM_003000): exon 1–8;
	17. SDHC (NM_003001): exon 1–6;
	18. SDHD (NM_003002): exon 1–4
SNPs target region chromosome 11	– rs2513613	– rs10838307
	– rs34593780	– rs4267090
	– rs2631403	– rs7939803
	– rs330253	– rs2887046
	– rs73455029	– rs11233227
	– rs7949600	– rs6483324
	– rs1247726	– rs2851171
	– rs4943948	– rs736287
	– rs681017	– rs2510718
	– rs10750552	– rs1638585
	– rs1620333	– rs7110021
	– rs630172	– rs35787427
	– rs481303	– rs611697
	– rs1455113
SNPs target region chromosome 22	– rs1970640	– rs2017869
	– rs3747031	– rs1894252
	– rs5996639	– rs956548
	– rs2285206	– rs2038010
	– rs2294206	– rs2143695
	– rs62636244	– rs5769583
	– rs17003592	– rs1296750
	– rs3884944	– rs6010046

NM and ENST are both available at http://www.ensembl.org.

DTC differentiated thyroid carcinoma, FFPE formalin-fixed, paraffin-embedded, SNPs single-nucleotide polymorphisms