Fig. 3.

LXR agonists exerts metabolism-independent effects. Influence of 1 µM T0901317 (T09) and 1 µM GW3965 (GW), respectively, on insulin secretion in the presence of 15 mM glucose (a, control), 10 mM arginine (b) and 50 nM GLP-1 (c). n = 4–10. The agonists had no significant effects in the presence of arginine or GLP-1. Inhibiting effect of 10 µM T0901317 (T09) and 10 µM GW3965 (GW), respectively, on insulin secretion in the presence of 15 mM glucose (d, control), 10 mM arginine (e) and 50 nM GLP-1 (f). n = 5–10. Administration of 10 µM T0901317 on ΔΨ in the presence of 30 mM glucose still reveals a strong depolarisation. g Representative recording of ΔΨ with administration of T0901317. Metabolic integrity is shown by a rapid depolarisation by 0.5 µM FCCP. h Summary of all experiments. n = 18. Experiments were performed with different islet cell clusters or islets from 3–10 mice. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001