a PAS stain of epithelia of mice duodenum, jejunum, ileum, and colon. Male mice at age of 5 weeks old (the body weight of the mice is within normal limits) were selected randomly (n = 6). b Goblet cell number of epithelia of mice duodenum, jejunum, ileum, and colon (n = 6). c Determination of mRNA level of Muc2 (n = 6). d Determination of Muc2 in mice intestinal epithelia cells by western blot by using Muc2(ab134119) antibody. e Immunofluorescent stain of mice intestinal epithelia with Muc2(H300) antibody. *P < 0.05; **P < 0.01; ***P < 0.001.